Journal: Journal of Oral Biology and Craniofacial Research
Article Title: Oral thin film containing rice husk liquid smoke effects on the expression of key cytokines and growth factors in a periodontitis model
doi: 10.1016/j.jobcr.2025.08.007
Figure Lengend Snippet: OTF containing rice husk liquid smoke treatment significantly increase BMP-2 expression in periodontal tissue. (A) Representative immunohistochemical staining images show BMP-2 expression at different magnifications after 2 and 7 days post-treatment. (B) Quantitative analysis confirms significantly elevated BMP-2 expression in the OTF-treated group at both time points. Statistical analysis was performed using one-way ANOVA followed by Tukey's HSD post hoc test (p < 0.05). Red arrow head indicated a positive cell for BMP-2 expression. Scale bar 100 μm.
Article Snippet: Primary antibodies used included IL-6 (sc-130326; mouse monoclonal, Santacruz biotechnology); IL-10 (sc-8438; mouse monoclonal, Santacruz biotechnology); FGF-2 (sc-74412; mouse monoclonal, Santacruz biotechnology), BMP2 (sc-137087; mouse monoclonal, Santacruz biotechnology) with an overnight incubation at 4 °C.
Techniques: Expressing, Immunohistochemical staining, Staining